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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which ends up in chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant position in marketing mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be described. Two supplemental splice variants ended up noted but in-depth nucleotide sequence facts is not really nevertheless accessible.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This tutorial outlines further doable optimization of mobile and immunoassay parameters to ensure the absolute best success are...
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This guidebook outlines even further probable optimization of cellular and immunoassay parameters to make certain the best possible benefits are...
Antibodies sure to the blocking peptide now not bind to the epitope within the focus on protein. By evaluating the staining from your blocked antibody versus the antibody alone, you can see which staining is particular.
Target Data Cyclin E belongs for the extremely conserved cyclin family members, whose associates are characterized by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation designs which contribute to your temporal coordination of each and every mitotic occasion. Cyclin E kinds a posh with and functions as a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates in the G1-S period boundary which is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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